The study’s objective is to determine the Sai River’s water quality, which is a sacred river in Uttar Pradesh, India. The river Sai, a
significant river in Uttar Pradesh, rises from a pond in the hamlet of Bijgwan, close to Pihani in the district of Hardoi. It flows for around
600 kilometers, forming the district border between Lucknow and Unnao. It ultimately meets the Gomati River near Rajepur in Jaunpur
district, having previously passed through Raebareli, Hardoi, and Jaunpur district. In the Indian state of Uttar Pradesh, the Sai River is a
tributary of the Gomti River. In UP, the Sai is revered as a sacred river. Three water samples in total from the Raebareli area
Bhanvareshvar Mandir Raebareli, Raj Ghat Raebareli, and Shaheed Smarak Raebareli were gathered, and their microbiological analyses
were utilized to determine the samples’ overall water quality index. The observational and significant technique used here is MPN (Most
Probable Number). Here the study will focus on the amount of Total Coliform (TC) Fecel Coliform (FC) and Fecel Streptococci, present in
the Holy water Sai and significantly discuss the Water Quality and health implications. Since water is the basic requirement for not only
humans but also for every living organism, Quality and purity given human health especially in view its microbial contamination.