Tomato (Solanum lycopersicum L.) is a vital horticultural crop with global significance, offering essential nutrients and economic value. Genetic variability plays a foundational role in breeding programs, providing the basis for improving traits such as fruit yield, size, and stress resistance. This study investigates genetic variability, heritability, and genetic advance in tomato to assess the scope of response to selection. High phenotypic and genotypic coefficients of variation (PCV and GCV) were observed for key traits, indicating substantial genetic diversity. Traits with high heritability and genetic advance, such as fruit yield, demonstrate strong potential for genetic improvement through selection. The findings emphasize the importance of integrating traditional breeding methods with molecular tools to enhance tomato cultivars, ensuring sustainable production and adaptability to environmental challenges. This research provides critical insights into optimizing genetic resources for tomato breeding programs aimed at improving productivity and quality.