Dharwad is one of the largest districts situated in the western sector of the northern half of Karnataka State. Karnatak University is a public state university in the Dharwad district of Karnataka state, India, and is spread across 888 acres. The survey was conducted in Karnatak University Campus in 2021 – 2022 for documenting tree diversity and assessing the carbon sequestration capacity. A total of 223 tree species belonging to 56 families and 158 genera were recorded in 80 quadrats. Among which Fabaceae has the highest number of species (41), followed by Malvaceae (16), Bignoniaceae (14), Moraceae (11), Apocynaceae, Meliaceae, and Rubiaceae (8) respectively. Total basal area of all the tree species in the campus is 70.704 m2. About 50,353 individuals were measured for calculating above-ground biomass, below-ground biomass, total biomass, and carbon storage capacity. The Importance Value Index was calculated using relative frequency, relative dominance, and relative density of all the tree species. Total carbon sequestered by all the tree species in the campus is about 3400.53 tons, of which Eucalyptus globulus sequestered highest 32.1 tons of carbon, followed by Tamarindus indica sequestered 3.31 tons of carbon, followed by Mangifera indica sequestered 2.77 tons of carbon, followed by Delonix regia sequestered 2.22 tons of carbon, which is followed by Azadirachta indica sequestered 1.77 tons of carbon being the top five tree species with highest carbon storage capacities. These trees are sequestering a good amount of carbon, hence keeping the campus less polluted and more cleaner air quality.