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The BLAST analysis is a fast method for identifying likely microbial species. However, it is not always able to definitively determine closely related genera or taxa because there may be numerous similar or identical scores from homologous sequences. In order to overcome this constraint, the utilization of phylogenetic tree construction and analysis is employed to get a more accurate molecular characterization of microorganisms. This study utilized the 16S rDNA sequence of BRSSOU1, which formed a cluster with a particular strain in the phylogenetic tree and exhibited a 99.92% alignment with Rhodopseudomonas sp. according to the NCBI-BLAST results. Therefore, BRSSOU1 was determined to be Rhodopseudomonas palustris. Our deduction is that BRSSOU1 is a variant of Rhodopseudomonas palustris. The strains were obtained from water samples collected at the source of the Jhelum River near Verinag, Kashmir. In addition, samples of wastewater were obtained from several rivers, namely Arpath, Lidder, Neelum, Sind, Kunhar, and Pohru. PNSB strains were isolated and identified using both traditional bacterial culture techniques and molecular approaches. The identification technique effectively uncovered these strains of Purple Non-sulfur Bacteria (PNSB).