The integrated application of mineral fertilizers and organic materials is widely recognized as an effective and eco-friendly strategy for achieving sustainable crop production. To evaluate the impact of nitrogen and sulphur supplementation through organic sources on nutrient content and uptake in tomato (Solanum lycopersicum), a field experiment was conducted during the Rabi season of 2019–20 at the Nursery Area Organic Farm, Karguwan Ji, Bundelkhand University, Jhansi, Uttar Pradesh. The study employed a Randomized Block Design (RBD) with nine treatment combinations and three replications. The findings revealed that the combined use of organic amendments significantly enhanced the nutrient content and uptake in tomato plants, underscoring the potential of organic nutrient sources in improving soil fertility and crop nutritional status under sustainable farming systems. The highest contents (N,P,K,S) were reported in T8 (50 % VC+50% FYM + 30 kg/ha S) with value of (2.63 %, 0.24 %, 2.16 %, 0.23 %) and least was reported in T4 N (1.20 %), TO P (0.11 %), T3 K (1.43 %), T2 S (0.18 %). Highest uptakes (N,P,K,S) was reported in T8 (119.32 kg/ha, 11.89 Kg/ha, 103.03 kg/ha, 11.34 kg/ha) and least was reported in T4 N (42.50 kg/ha), TO P (5.04 kg/ha), T3 K (58.13 kg/ha), T2 S (7.02 kg/ha).