Nanoparticles derived from plants hold immense promise across disciplines such as agriculture and medicine due to their antimicrobial and anticancer capabilities. So, from rising concerns due to their potential adverse effects—commonly referred to as nanotoxicity—which may lead to phytotoxic responses in crops and pose ecological risks through accumulation in biological systems. This method utilizes Zn (NO₃) ₂.6H₂O and bioactive compounds from the Aqueous leaf extract, ensuring a non-toxic process. The Integrated samples were thoroughly characterized using various techniques, including UV-visible spectroscopy, FTIR, XRD, EDX, Zeta potential analysis, SEM, TEM, and DLS, all of which confirmed their structure and stability. The formation of nanoparticles was confirmed by UV-visible spectroscopy, which displayed a characteristic surface plasmon resonance (SPR) band at 374 nm. Furthermore, FTIR analysis revealed the presence of key functional groups, including those corresponding to alcohols, carboxylic acids, and amine salts. isothiocyanates, aromatic compounds, conjugated alkenes, aldehydes, and halo compounds, highlighting the role of bioactive components in nanoparticle synthesis. XRD analysis confirmed a spherical crystalline structure, while EDX analysis determined the elemental composition of Zinc (41.8%), Nitrogen (14.7%), and Oxygen (38.4%). SEM analysis measured the nanoparticle size at 40 nm, with a potential Zeta value of +6.6 mV and DLS measurement of 145.2 nm, ensuring stability and uniformity. The exhibited antioxidant, antimicrobial, antifungal, anti-diabetic, and anti-cancer properties, demonstrating their potential for biomedical applications. Their effectiveness against Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus flavus, and Pichia anomala is notable, with anti-bacterial and anti-fungal properties. The potential therapeutic relevance of these materials is highlighted by their anti-cancer activity observed against the MCF-7 and A549 cell lines. This investigation details a sustainable and cost-effective approach for the eco-friendly synthesis of Zinc Oxide Nanoparticles (ZnO NPs) using an aqueous leaf extract of Hibiscus lobatus. Ultimately, these results indicate that the plant-mediated compounds could function as viable, promising alternatives within nanomedicine, thereby fostering progress in healthcare and biomedical research.