This review delves into the extensive body of ethnobotanical research on medicinal plants used in traditional medicine across South Africa. These plants hold significant cultural value and are employed to treat a wide array of ailments, showcasing the rich heritage of indigenous knowledge passed down through generations. The review synthesizes findings from numerous studies, offering a comprehensive overview of the therapeutic applications and preparation methods of these medicinal plants. It underscores the importance of preserving this indigenous knowledge, not only for cultural heritage but also for its potential integration into modern healthcare systems to improve health outcomes. Furthermore, the review addresses the critical challenges faced by traditional medicine, including overharvesting of plant resources, biodiversity loss, and the erosion of cultural knowledge due to modernization and urbanization. By discussing these challenges, the review advocates for sustainable harvesting practices, conservation strategies, and the creation of frameworks that facilitate the respectful integration of traditional medicine into contemporary health practices. This comprehensive analysis aims to highlight the value of traditional medicinal plants and the urgent need for collaborative efforts to ensure their preservation and sustainable use.