This study aimed to explore the pharmacological effects of Lentinus edodes, focusing on its potential for large-scale cultivation and hypocholesterolemic properties. Research efforts concentrated on developing cultivation technologies to enhance biological efficiency and evaluating the impact of L. edodes on lipid profiles. Results showed a significant reduction in serum total cholesterol, which increased with higher supplementation levels and prolonged administration. Additionally, L. edodes supplementation raised HDL levels and lowered LDL, triglycerides, and total lipid levels. These findings suggest that L. edodes holds promise as a therapeutic agent for cholesterol management and as a valuable crop for medicinal cultivation.