Bacterial proteases, a diverse group of enzymes produced by various bacterial species, hold significant potential in biotechnology due to their unique properties and wide range of applications. These enzymes catalyze the hydrolysis of proteins, and their functionality under various conditions makes them highly valuable in industrial processes. The biotechnological prospects of bacterial proteases encompass several industries, including pharmaceuticals, food, leather, and detergents. In the pharmaceutical industry, bacterial proteases are instrumental in the synthesis of peptide-based drugs and the development of novel therapeutic agents. Their specific action on proteins is utilized in drug formulation and delivery systems. In the food industry, these enzymes play a crucial role in processing proteins, modifying food texture, and enhancing flavor profiles. They are also used in the production of protein-rich dietary supplements. The leather industry benefits from bacterial proteases in the eco-friendly processing of hides, offering a sustainable alternative to chemical-based methods. In detergent formulations, these enzymes contribute to effective stain removal at various temperature and pH ranges, enhancing cleaning efficiency. Recent advancements in biotechnology, including recombinant DNA technology and protein engineering, have further expanded the scope of bacterial protease applications. These technologies enable the enhancement of enzyme properties, such as stability, specificity, and activity under extreme conditions, making them more suitable for industrial applications.